bdec human primary bladder epithelial cells  (ATCC)

Journal: Cell Death and Differentiation

Article Title: TTK promotes mitophagy by regulating ULK1 phosphorylation and pre-mRNA splicing to inhibit mitochondrial apoptosis in bladder cancer

doi: 10.1038/s41418-025-01492-w

Figure Lengend Snippet: A Relative expression of TTK in normal bladder tissues and tumor tissues in the TCGA BLCA and GSE13507 datasets. B Kaplan–Meier survival curves showing the overall survival of patients with low and high TTK expression from the TCGA BLCA and GSE13507 datasets. C Western blot analysis of TTK expression in BC tissues and adjacent normal tissues (n = 24). Densitometric and statistical analysis. D Immunohistochemical staining showing TTK expression levels in BC tissues and corresponding adjacent tissues (original magnification, ×400), quantitatively rated using histochemical scoring (n = 20). (E and F) TTK mRNA and protein expression levels in BC cell lines (TCCSUP, T24, 5637, RT-112, UM-UC-3, and J82) and the primary bladder epithelial cell line BdEC as detected by qRT-PCR ( E ) and Western blot ( F ). Data are expressed as mean ± SD from three independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: The primary normal bladder epithelial cell line, BdEC (#PCS-420-010, ATCC, USA), was cultured in bladder epithelial cell basal medium supplemented with bladder epithelial growth factors.

Techniques: Expressing, Western Blot, Immunohistochemical staining, Staining, Quantitative RT-PCR

Journal: Advanced Healthcare Materials

Article Title: Dual‐Layer Nanoengineered Urinary Catheters for Enhanced Antimicrobial Efficacy and Reduced Cytotoxicity

doi: 10.1002/adhm.202401700

Figure Lengend Snippet: Evaluation of the antibacterial properties and cytocompatibility of the nanoengineered urinary catheters. a) Optical images of colonies on agar plates and the average colony counts for E. coli and S. aureus . The antibacterial effect of Zn/AgNP‐coated catheters is significantly enhanced (one‐way ANOVA test; n = 6 per group; error bars showed standard deviations; * p < 0.05, ** p < 0.01, and **** p < 0.001). b) Fluorescently stained cellular morphologies and cell viability of human dermal fibroblasts (HDFs) and bladder epithelial cells (BdECs) after 1 d of culturing in extracts (one‐way ANOVA test; n = 6 per group; error bars showed standard deviations; **** p < 0.001).

Article Snippet: HDFs (PCS‐201‐012, ATCC, USA) and BdECs (PCS‐420‐010, ATCC, USA) were utilized to assess the cytotoxicity of the samples.

Techniques: Staining